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CiPA Cardiac Ion Channels
- Assays and cell lines -
- B’SYS is a specialty provider for cardiac safety studies and CiPA - Comprehensive in vitro Proarrhythmia Assays
- Member of the "CiPA Ion-Channel Work-Group" (FDA/HESI CiPA initiative)
- 15 years’ experience in conducting ion channel studies
- With our experienced team and array of capabilities we can support screening of compounds from discovery to entry-into-man / IND enabling
- Proprietary cardiac ion channel cell lines
- Cardiac safety studies on three independent automated patch clamp rigs (Sophion QPatch™)
- Ion channel trafficking studies (current density and luminescence assays)
- CiPA compliant action potential studies on iPS derived cardiomyocytes(Nanion CardioExcyte™)
- Customer oriented study design
|
Channel / Reference Compound / Cell Line |
Representative Current recorded using CiPA Voltage Protocol |
Assay |
|
NaV1.5 peak current
NP_000326.2
Mexilitine, IC50: 48.49 µM Cell line: CHO/ HEK293 |
 |
Manual
Automated
GLP
|
|
NaV1.5 late current
NP_000326.2
Ranolazine, IC50: 18.51 µM 30 nM ATXII Cell line: CHO / HEK293 |
 |
Manual
Automated
GLP
|
|
CaV1.2
AAA51900.1, AAL16948.1, NP_001005505.1
Isradipine, IC50: 17.48 nM
Cell line: HEK293 |
 |
Manual
Automated
GLP
|
|
hERG (IKr)
NP_000229.1
Odansetron, IC50: 1171.47 nM
Cell line: CHO / HEK293 |
 |
Manual
Automated
GLP
|
|
KVLQT1 / minK (IKs)
NP_000209.2, NP_000210.
Bepridil, IC50: 13.88 µM
Cell line: CHO / HEK293 |
 |
Manual
Automated
GLP
|
|
KV4.3 / KChip2 NP_004971, NP_775283.1
Diltiazem, IC50: 34.68 nM
Cell line: CHO |
 |
Manual
Automated
GLP
|
|
Kir2.1
NP_000882
ML-113, IC50: 176.95 nM
Cell line: CHO |
 |
Manual
Automated
GLP
|
