Patch clamping

B‘SYS is equipped with state of the art patch-clamp technology. Our setups are equipped with systems for rapid drug application.

Further options for your experiments:

  • Full glass equipment
  • Temperature control
  • Full human plasma or in the presence of BSA or other relevant proteins

Application schemes and assay conditions can be adapted for your needs.

Depending on the application scheme  and the type of ion channel the manual setups at B'SYS allow the measurement of 20-50 compounds per day.

Whole-cell recordings or single-channel analysis in various expression systems (HEK 293, CHO, Neuroblastoma etc.) can be conducted to test the effects of drugs on voltage-gated or ligand-gated ion channels.

B'SYS currently operates 3 automated patch clamp devices (Sophion Qpatch™).

Please contact us for details or offers: [email protected]

For details, please check our catalog.

 

Voltage gated ion Channels (VGIC)

CiPAPotassium HCN Sodium Calcium Chloride TRP

 

Ligand gated ion receptors

GABAA Glutamate Acetylcholine P2X Glycine Serotonine Acid sensing

 

Cardiac Panel / Comprehensive in Vitro Proarrhytmia Assay (CiPA)

CiPa

 

 

Voltage gated ion channel (VGIC)

 

 

Potassium Channels

Channel Gene Manual Automated
KV1.1 KCNA1  Available Available
KV1.2 KCNA2  Available Available
KV1.3 KCNA3 Available Available
KV1.4 KCNA4  Available Available
KV1.5 KCNA5 Available Available
KV1.6 KCNA6  Available Available
KV1.7 KCNA7 Available Available
KV2.1 KCNB1 Available Available
KV2.2 KCNB2 Available Available
KV3.1 short KCNC1 Available Not available yet
KV3.1 long KCNC1 Available Not available yet
KV3.2 KCNC2 Available Not available yet
KV3.3 KCNC3 Available Not available yet
KV3.4 KCNC4 Available Not available yet
KV4.2 kCND2 Available Not available yet
KV4.3 KCND3 Available Available
KV4.3 / KChiP KCND3 / KCNIP2 Available Available
KV7.1 KCNQ1 Available Not yet available
KV7.1 / minK KCNQ1 / MINK  Available Available
KV7.2 KCNQ2 Available Not available yet
KV7.3 KCNQ3 Available Not yet available
KV7.2 / 7.3 KCNQ2/3  Available Not yet available
KV7.3 / 7.5 KCNQ3/5  Available Not yet available
KCa2.1 KCNN1 Available Not yet available
KCa3.1 KCNN4 Available Not yet available
KCa4.1 KCNT1 Available Not yet available
K2P1.1 KCNK1 Available Not yet available
K2P18.1 KCNK18 Available Not yet available
KV11.1 (hERG) KCNH2 Available Available
hERG Duo KCNH2 Available Available
Kir1.1 KCNJ2 Available Available
Kir2.1 KCNJ1, KIR1.1, ROMK, ROMK1 Available Available
Kir2.2 KCNJ12 Available Not yet available
Kir2.3 KCNJ4 Available Not yet available
BK KCNMA1 Available Not yet available
Neuroblastoma whole potassium channels (N1E-115)  Available Available

 

HCN Channels

Channel Gene Manual Automated
HCN1 HCN1 Available Available
HCN2 HCN2 Available Available
HCN3 HCN3 Available Not yet available
HCN4 HCN4 Available Available

 

Sodium Channels

Channel Gene Manual Automated
NaV1.1 SCN1A  Available Available
NaV1.2 SCN2A  Available Available
NaV1.3 SCN3A  Available Available
NaV1.4 SCN4A  Available Available
NaV1.5 SCN5A  Available Available
NaV1.5 big late SCN5A  Available Available
NaV1.6 SCN8A  Available Available
NaV1.7 SCN9A  Available Available
NaV1.8 SCN10A  Available Available
NaV1.9 SCN11A  Available Available
Neuroblastoma whole sodium (TTX sensitive sodium channels)  Available Available

 

Calcium Channels

Channel Gene Manual Automated
CaV1.2  CACNA1C / CACNB2 / CACNA2D Available Available
CaV1.3  CACNA1D / CACNB3 / CACNA2D Available Available
CaV2.1  CACNA1A / CACNB3 / CACNA2D2 or CACNA2D4  Available Not yet available
CaV3.1 CACNA1G Available Not yet available
CaV3.2  CACNA1H  Available Available
CaV3.3  CACNA1I Available Not yet available
Neuroblastoma whole calcium (T- and L- type)  Available Available

 

Chloride Channels

Channel Gene Manual Automated
ClC-2  CLCN2 Available Not yet available
CFTR CFTR Available Available

 

TRP Channels

Channel Gene Manual Automated
TRPA1  TRPA1 Available Not yet available
TRPM4  TRPM4 Available Not yet available
TRPM8  TRPM8 Available Available
TRPV1  TRPV1 (VR1) Available Available
mTRPV1 (mouse) Trpv1 Available Not yet available
TRPV2 TRPV2 Available Not yet available
TRPV3 TRPV3 Available Not yet available
TRPV4 TRPV4 coming soon coming soon

 

 

Ligand gated ion receptors

 

GABAA Receptors

Channel Gene Manual Automated
GABAA (α1β2γ2)  GABRA1 / GABRB2 / GABRG2 Available Available
GABAA (α1β3γ2)  GABRA1 / GABRB3 / GABRG2 Available Available
GABAA (α2β2γ2) GABRA2 / GABRB2 / GABRG2 Available Available
GABAA (α3β2γ2)  GABRA3 / GABRB2 / GABRG2 Available Available
GABAA (α4β3d) GABRA4 / GABRB3 / GABRD Available Not yet available
GABAA (α5β2γ2)  GABRA5 / GABRB2 / GABRG2 Available Available
GABAA (α6β3d)   GABRA6 / GABRB3 / GABRD Available Not yet available
GABAA (β3) GABRB3 Available Available

 

Glutamate Receptors

Channel Gene Manual Automated
AMPA  GRIA1 Available Not yet available
Kainate K1 GRIK1 Available Not yet available
Kainate K2 GRIK2 Available Available
Kainate K2/K5 GRIK2/5 Available Not yet available
NMDA (NR1 / 2A)  GRIN1 / GRIN2A Available Available
NMDA (NR1 / 2B)  GRIN1 / GRIN2B Available Available
NMDA (NR1 / 2C) GRIN1 / GRIN2C Available Not yet available
NMDA (NR1 / 2D) GRIN1 / GRIN2D Available Not yet available

 

Acetylcholine Receptors

Channel Gene Manual Automated
nAChR (α7)  CHRNA7 Available Not yet available
nAChR (α3β4)  CHRNA3 / CHRNB4 Available Not yet available
nAChR (α4β2)  CHRNA4 / CHRNB2 Available Available

 

P2X Receptors

Channel Gene Manual Automated
P2X1 P2RX1 Available Not yet available
P2X2 P2RX2 Available Available
P2X3 P2RX3 Available Not yet available
P2X2/3 P2RX2/P2RX3 Available Not yet available
P2X4 P2RX4 Available Available
P2X7 P2RX7 Available Available

 

Glycine Receptors

Channel Gene Manual Automated
GlyRα1 GLYRA1 Available Not yet available
GlyRα2 GLYRA2 Available Not yet available
GlyRα3 GLYRA3 Available Available
GlyRα3β GLYRA3B Available Not yet available

 

Serotonine Receptors

Channel Gene Manual Automated
5HT3A 5HT3A Available Available
5HT3A/B 5HT3A / 5HT3B Available Not yet available

 

Acid sensing Receptors

Channel Gene Manual Automated
ASIC1 isoform 1 (1a long) ACCN2 Available Available
ASIC1 isoform 2 (1a short) ACCN2 Available Available
ASIC1 isoform 3 (1b) ACCN2 Available Available
ASIC2 ACCN1 Available Not yet available
ASIC3 ACCN3 Available Available