CiPA

Patch clamping

B‘SYS is equipped with state of the art patch-clamp technology. Our setups are equipped with systems for rapid drug application (Dyna Flow Y Rapid Solution Charger).

Further options for your experiments:

  • Full glass equipment
  • Temperature control
  • Full human plasma or in the presence of BSA or other relevant proteins

Application schemes and assay conditions can be adapted for your needs.

Depending on the application scheme  and the type of ion channel the manual setups at B'SYS allow the measurement of 20-50 compounds per day.

Whole-cell recordings or single-channel analysis in various expression systems (HEK 293, CHO, Neuroblastoma etc.) can be conducted to test the effects of drugs on voltage-gated or ligand-gated ion channels.

Please contact us for details or offers: info@bsys.ch

For details, please check our catalog.

 

Voltage gated ion channels (VGIC)

CiPAPotassium HCN Sodium Calcium Chloride TRP

 

Ligand gated ion receptors

GABAA Glutamate Acetylcholine P2X Glycine Serotonine Acid sensing

 

 

Potassium Channels

ChannelGene Manual Automated
KV1.1 KCNA1  Available Available
KV1.2 KCNA2  Available Available
KV1.3 KCNA3
Available Available
KV1.4 KCNA4  Available Available
KV1.5 KCNA5 Available Available
KV1.6 KCNA6  Available Available
KV2.1 KCNB1 Available Available
KV2.2 KCNB2 Available Available
KV4.3 KCND3 Available Available
KV4.3 / KChiP KCND3 / KCNIP2 Available Available
KV7.1 KCNQ1 Available Not yet available
KV7.1 / minK KCNQ1 / MINK  Available Available
KV7.2 KCNQ2 Available Not available yet
KV7.3 KCNQ3 Available Not yet available
KV7.2 / 7.3 KCNQ2/3  Available Not yet available
KCa2.1 KCNN1 Available Not yet available
KCa3.1 KCNN4 Available Not yet available
K2P1.1 KCNK1 Available Not yet available
K2P18.1 KCNK18 Available Not yet available
KV11.1 (hERG) KCNH2 Available Available
hERG Duo KCNH2 Available Available
Kir2.1 KCNJ2 Available Available
Kir3.1 KCNJ3 Available Not yet available
Kir3.4 KCNJ5 Available Not yet available
Neuroblastoma whole potassium channels (N1E-115)  Available Available

 

HCN Channels

ChannelGene Manual Automated
HCN1 HCN1 Available Available
HCN4 HCN4 Available Available

 

Sodium Channels

ChannelGene Manual Automated
NaV1.1 SCN1A  Available Available
NaV1.2 SCN2A  Available Available
NaV1.3 SCN3A  Available Available
NaV1.4 SCN4A  Available Available
NaV1.5 SCN5A  Available Available
NaV1.6 SCN8A  Available Available
NaV1.7 SCN9A  Available Available
NaV1.8 SCN10A  Available Available
Neuroblastoma whole sodium (TTX sensitive sodium channels)  Available Available

 

Calcium Channels

ChannelGene Manual Automated
CaV1.2  CACNA1C / CACNB2 / CACNA2D Available Available
CaV1.3  CACNA1D / CACNB3 / CACNA2D Available Available
CaV2.1  CACNA1A / CACNB3 / CACNA2D2 or CACNA2D4  Available Not yet available
CaV3.1 CACNA1G Available Not yet available
CaV3.2  CACNA1H  Available Available
CaV3.3  CACNA1I Available Not yet available
Neuroblastoma whole calcium (T- and L- type)  Available Available

 

Chloride Channels

ChannelGene Manual Automated
ClC-2  CLCN2 Available Not yet available
CFTR CFTR Available Available

 

TRP Channels

ChannelGene Manual Automated
TRPA1  TRPA1 Available Not yet available
TRPM4  TRPM4 Available Not yet available
TRPM8  TRPM8 Available Available
TRPV1  TRPV1 (VR1) Available Available
mTRPV1 (mouse) Trpv1 Available Not yet available
TRPV2 TRPV2 Available Not yet available
TRPV3 TRPV3 Available Not yet available
TRPV4 TRPV4 Available Not yet available

 

 

Ligand gated ion receptors

 

GABAA Receptors

ChannelGene Manual Automated
GABAA 1β2γ2)  GABRA1 / GABRB2 / GABRG2 Available Available
GABAA 1β3γ2)  GABRA1 / GABRB3 / GABRG2 Available Available
GABAA 2β2γ2) GABRA2 / GABRB2 / GABRG2 Available Available
GABAA 3β2γ2)  GABRA3 / GABRB2 / GABRG2 Available Available
GABAA 4β3d)
GABRA4 / GABRB3 / GABRD Available Not yet available
GABAA 5β2γ2)  GABRA5 / GABRB2 / GABRG2 Available Available
GABAA 6β3d)   GABRA6 / GABRB3 / GABRD Available Not yet available
GABAA (β3) GABRB3 Available Available

 

Glutamate Receptors

ChannelGene Manual Automated
AMPA  GRIA1 Available Not yet available
Kainate K1 GRIK1 Available Not yet available
Kainate K2 GRIK2 Available Available
Kainate K2/K5 GRIK2/5 Available Not yet available
NMDA (NR1 / 2A)  GRIN1 / GRIN2A Available Available
NMDA (NR1 / 2B)  GRIN1 / GRIN2B Available Available
NMDA (NR1 / 2C) GRIN1 / GRIN2C Available Not yet available
NMDA (NR1 / 2D) GRIN1 / GRIN2D Available Not yet available

 

Acetylcholine Receptors

ChannelGene Manual Automated
nAChR (α7 CHRNA7 Available Not yet available
nAChR (α4β2 CHRNA4 / CHRNB2
Available Available

 

P2X Receptors

ChannelGene Manual Automated
P2X1 P2RX1 Available Not yet available
P2X2 P2RX2 Available Available
P2X3 P2RX3 Available Not yet available
P2X4 P2RX4 Available Available
P2X7 P2RX7 Available Available

 

Glycine Receptors

ChannelGene Manual Automated
GlyRα3
GLYRA3 Available Available
GlyRαβ3
GLYRA3B Available Not yet available

 

Serotonine Receptors

ChannelGene Manual Automated
5HT3A 5HT3A Available Available
5HT3A/B 5HT3A / 5HT3B Available Not yet available

 

Acid sensing Receptors

ChannelGene Manual Automated
ASIC1 isoform 1 (1a long) ACCN2 Available Available
ASIC1 isoform 2 (1a short) ACCN2 Available Available
ASIC1 isoform 3 (1b) ACCN2 Available Available
ASIC2 ACCN1 Available Not yet available
ASIC3 ACCN3 Available Available