CiPA

Swiss Quality in Ion channel Services

CiPA Cardiac Ion Channels

- Assays and cell lines –

  • B’SYS is a specialty provider for cardiac safety studies and CiPA - Comprehensive in vitro Proarrhythmia Assays
  • Member of the "CiPA Ion-Channel Work-Group" (FDA/HESI CiPA initiative)
  • 15 years’ experience in conducting ion channel studies
  • With our experienced team and array of capabilities we can support screening of compounds from discovery to entry-into-man / IND enabling
  • Proprietary cardiac ion channel cell lines
  • Cardiac safety studies on three independent automated patch clamp rigs (Sophion QPatch™)
  • Ion channel trafficking studies (current density and luminescence assays)
  • CiPA compliant action potential studies on iPS derived cardiomyocytes(Nanion CardioExcyte™)
  • Customer oriented study design

 

Channel / Reference Compound / Cell Line

Representative Current recorded using

CiPA Voltage Protocol

Assay

NaV1.5 peak current

 

NP_000326.2

 

Mexilitine, IC50: 48.49 µM


Cell line: CHO/ HEK293 

CIPA Nav1.5 peak current

                             Manual

Available

Automated

Available

GLP

Available

NaV1.5 late current

 

NP_000326.2

 

Ranolazine, IC50: 18.51 µM

30 nM ATXII

Cell line: CHO / HEK293

CIPA Nav1.5 late current

Manual

Available

Automated

Available

GLP

Available

CaV1.2

 

AAA51900.1, AAL16948.1, NP_001005505.1

 

Isradipine, IC50: 17.48 nM

 

Cell line: HEK293

CIPA Cav1.2

Manual

Available

Automated

Available

GLP

Available

hERG (IKr)

 

NP_000229.1

 

Odansetron, IC50: 1171.47 nM

 

Cell line: CHO / HEK293

CIPA herg L

Manual

Available

Automated

Available

GLP

Available

KVLQT1 / minK (IKs)

 

NP_000209.2, NP_000210.

 

Bepridil, IC50: 13.88 µM

 

Cell line: CHO / HEK293

CIPA KV LQT

Manual

Available

Automated

Available

GLP

Available

KV4.3 / KChip2


NP_004971, NP_775283.1

 

Diltiazem, IC50: 34.68 nM

 

Cell line: CHO

CIPA KV4.3 Chip

Manual

Available

Automated

Available

GLP

Not available yet

Kir2.1

 

NP_000882

 

ML-113, IC50: 176.95 nM

 

Cell line: CHO

CIPA Kir2.1

Manual

Available

Automated

Available

GLP

Not available yet